Case Summary:

Guided Tissue Regeneration (GTR) is a technique performed to promote osseous regeneration, allowing for tissue growth (bone and soft tissues) to replace an infrabony periodontal pocket (a site of vertical bone loss). This involves cleaning the infrabony pocket using hand curettes, potentially with a specialized ultrasonic curette as well. A bone graft, along with a barrier membrane, is placed into the pocket, followed by tight closure of the gingival collar. GTR is performed to preserve structural integrity in cases of vertical bone loss.

Patient Information:

Buffy, a 6yo FS 11# Beagle presented from the referring veterinarian (RDVM), Dr. Ryan Southard, at Family Pet Veterinary Center, for a Comprehensive Oral Health Assessment and Treatment (COHAT). Initial exam findings calculus index 1-3 in varied areas of the mouth; 409 gingival recession (GR) 4mm with exudate at the furcation.

Dental Exam Findings:

PD2 (Periodontal Disease Index <25% attachment loss) 102-103, 202-203, 104

PD3 (Periodontal Disease Index 25-50% attachment loss) 204

FE2 (Furcation Exposure under the crown) GR (Gingival Recession) 409, 4mm

Missing 105-109, 201, 205-207, 306-308, 302-303, 401-403, 405, 408


Advanced Imaging with RDVM:

Below: CBCT Hard tissue reconstruction

CBCT Hard tissue reconstruction with RDVM

Below: High intensity CBCT Image

High intensity CBCT Image with RDVM

Below: Bone loss affecting upper left canine tooth

Bone Loss affecting uppler left canine tooth

Below: Imaging of both sides of the lower right 4th premolar

Furcation exposure level 2 indicating bone loss under the crown

Both sides of the lower right 4th premolar - Furcation exposure through and through - Bone loss
Procedures Performed:


Closed Root Planning (RP/C):

During the dental procedure Dr. Ryan noted probing depth of 8mm at the palatal aspect 204 and 5mm palatal probing depth of 104. Closed root planing (RP/C) was performed at this time and referred for Guided Tissue Regeneration (GTR).

Below: Presentation at the beginning of procedure with RDVM

Presentation at the beginning of procedure with RDVM

Below: After procedure with RDVM (Before referral to Animal Dentistry Referral Services)

After procedure with RDVM

In the one month after the referring DVM performed closed root planing, the upper right canine palatal pocket had improved from 5mm to 3mm (within normal limits) and the upper left canine infra-bony pocket depth had also improved from 8mm to 6mm. It’s typical to have a 1-2mm improvement in probing depth in the short term (1-3 months). Closed root planing turns an active periodontal pocket inactive. Recurrence of active periodontal disease is dependent up on pocket depth and dental home care as well as sooner (3-6 months) professional anesthetic dental procedures.

Guided Tissue Regeneration (GTR) 204:

An envelope flap was utilized, followed by curettage to remove granulation tissue and debris. Osteoallograft periomix bone graft material from Veterinary Transplant Services was then placed in the infrabony pocket. Doxirobe was spread thinly to create a membrane covering the graft material, and closure was achieved using 4-0 monocryl to create a tight gingival collar.

The images below were taken at various stages throughout the procedure.


CT imaging

Showing deep bone loss at palatal aspect of upper left canine tooth before an oral nasal fistula has developed

CT image after GTR done

Opening the defect site

View of the granulation bed which must be fully removed to be able to treat

gtr - before graft placement, granulation tissue from previous closed root planing from rdvm

Cleaned deep bony pocket

Also showing some debris, a good lesson to use magnification and clean more

gtr - site cleaned and ready for bone graft

Bone graft

Mixed with blood during GTR procedure

GTR during procedure - bone graft placed

Custom membrane placed over graft

To prevent soft tissue down growth and the bone returns

GTR - after bone graft placed, membrane on top

Closed GTR

With tight gingival collar

After suture placed after GTR perfomred


Surgical Extraction 409:

Envelope flap, section, elevate, graft placement. The site was closed with4-0 monocryl.

Below: (left) BEFORE extraction, (right) AFTER extraction

Left - Radiograph After Extraction


Follow-Up Information:

Buffy is a regular client and is doing great. Owner has no concerns and continues to see us regularly for the bone graft study. The most recent dental examination under anesthesia was September of 2023. Dental procedure findings: PP 0-1m 104, 204 with no bleeding on probing ie GTR is looking great, ie bone has returned to the area and attachment loss has been regained. Next dental procedure scheduled June 2024.


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